LOCUS       sequence-84921-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..51
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     2..22
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..806
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     misc_feature    807..872
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
       61 aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggggcaat
      121 cgaagcatgg gctaaaggag gagatgacgg tgaagtacca catggaaggt tgcgttaatg
      181 gtcacaaatt tgtcattact ggagagggca ttggaaaccc ttttaagggt aagcaaactg
      241 caaatttgtg tgtgatagaa ggaggcccgc tgccgttctc ggaggacatt ttaagtccgg
      301 gttttaaata tggtgaccgg attttcacag agtacccaca ggatattgta gattacttca
      361 agaactcatg tccagcgggc tatacgtggg aaaggagctt cctctttgag gacggagcgg
      421 tctgtcgatg caacgtggac ataacagtct ctgaaaagga gaactgcatc tatcacaaaa
      481 gtatcttcag aggggtgaat tttcccgccg acggccccgt aatgaagaaa atgaccacta
      541 attgggaagc tagtaccgag aaaattgtgc ctgttccaaa gcaagggata ttaaaaggaa
      601 aggtcaaaat gtacctattg ctgaaggatg gcggtcgtta tcattgccag tttgatacgg
      661 tatataaagc taaatcagtg ccctccaaga tgccagaatg gcattttata cagcataagc
      721 tccttaggga ggaccgctct gatgctaaaa accagaaatg gcaattaaca gaacatgcaa
      781 tcgcaggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
      841 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      901 gccataccac atttgtagag gttttacttg ctttaaaaac ctccacacct ccccctgaac
      961 ctgaaacata aatgaatgca attgtgtgtt aact
//
