LOCUS       sequence-84922-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 
            516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, 
            Liu Y, Zhang J, Xu P, Xu T
  TITLE     Rational design of true monomeric and bright photoactivatable 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021.
  PUBMED    22581370
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-05-13- 13"; volume: "9"; 
            issue: "7"; pages: "727-9"
COMMENT     Sequence Label: mEos3.2-N1
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..53
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     4..24
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    76..156
                     /label=MCS
                     /note="multiple cloning site"
     CDS             164..844
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
       61 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
      121 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgagtg cgattaagcc
      181 agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      241 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      301 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      361 ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg
      421 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg ccagaaacga
      481 cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc
      541 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      601 gtatgtgcgt gatggagtgc tgacgggtga tattgagatg gctttgttgc ttgaaggaaa
      661 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      721 accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
      781 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
      841 ataagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
      901 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgtt
//
