LOCUS       sequence-84924-e        1537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: tdEos-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..74
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             96..773
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             822..1499
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgggcg tggccgactt gatcaagaag ttcgagtcca
       61 tctccaagga ggagggggat ccaccggtcg ccaccatgag tgcgattaag ccagacatga
      121 agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
      181 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
      241 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
      301 ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
      361 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
      421 tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg
      481 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
      541 gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt
      601 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
      661 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
      721 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggta
      781 gcggtcacgg tactggttct actggttctg gttcttctga gagtgcgatt aagccagaca
      841 tgaagatcaa cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag
      901 atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg
      961 gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat
     1021 tcgccgaata tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt
     1081 cgtgggaacg aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa
     1141 caatggaagg ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca
     1201 atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg
     1261 tgcgtgatgg agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc
     1321 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag
     1381 gctaccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg
     1441 ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgataag
     1501 cggccgcgac tctagatcat aatcagccat accacat
//
