LOCUS       sequence-84925-e        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG
  TITLE     Time-lapse two-color 3D imaging of live cells with doubled 
            resolution using structured illumination.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5311-5. doi: 
            10.1073/pnas.1119262109. Epub 2012 Mar 19.
  PUBMED    22431626
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1119262109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-04-3- 3"; volume: "109"; issue: "14"; pages: "5311-5"
COMMENT     Sequence Label: tdTomato-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..74
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..1526
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgggcg tggccgactt gatcaagaag ttcgagtcca
       61 tctccaagga ggagggggat ccaccggtcg ccaccatggt gagcaagggc gaggaggtca
      121 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
      181 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      241 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
      301 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
      361 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
      421 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
      481 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
      541 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
      601 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
      661 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
      721 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg
      781 gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg
      841 ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
      901 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      961 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     1021 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     1081 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     1141 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     1201 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1261 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     1321 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     1381 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     1441 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     1501 acggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     1561 ac
//
