LOCUS       sequence-84930-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: HcRed1-N1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        65..164
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     115..135
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    187..267
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             275..961
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 agcggttgac tcacggggat ttcaagtctc cacccattga cgtcaatggg agtttgtttt
       61 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      121 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      181 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
      241 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcggcctgc tgaaggagag
      301 tatgcgcatc aagatgtaca tggagggcac cgtgaacggc cactacttca agtgcgaggg
      361 cgagggcgac ggcaacccct tcgccggcac ccagagcatg agaatccacg tgaccgaggg
      421 cgcccccctg cccttcgcct tcgacatcct ggccccctgc tgcgagtacg gcagcaggac
      481 cttcgtgcac cacaccgccg agatccccga cttcttcaag cagagcttcc ccgagggctt
      541 cacctgggag agaaccacca cctacgagga cggcggcatc ctgaccgccc accaggacac
      601 cagcctggag ggcaactgcc tgatctacaa ggtgaaggtg cacggcacca acttccccgc
      661 cgacggcccc gtgatgaaga acaagagcgg cggctgggag cccagcaccg aggtggtgta
      721 ccccgagaac ggcgtgctgt gcggccggaa cgtgatggcc ctgaaggtgg gcgaccggca
      781 cctgatctgc caccactaca ccagctaccg gagcaagaag gccgtgcgcg ccctgaccat
      841 gcccggcttc cacttcaccg acatccggct ccagatgctg cggaagaaga aggacgagta
      901 cttcgagctg tacgaggcca gcgtggcccg gtacagcgac ctgcccgaga aggccaacta
      961 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1021 taaaaacctc ccacacctcc ccctgaacct gaaacataaa tgaatgcatt gttgttgtaa
     1081 cttgtttatt gcagcttata tggtacaata aagcatagca tcacaatttc acaata
//
