LOCUS       sequence-84931-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKOet-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    27..107
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             142..792
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
       61 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
      121 agcaagggcg aggagaataa catggccgtg attaaaccag agatgaagat gaggtactac
      181 atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac aggcagacct
      241 tacgagggac atcaagagat gacactacgc gtcacaatgg ccaagggcgg gccaatgcct
      301 ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagaccttt tactaaatat
      361 ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg
      421 tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag ccttagagga
      481 aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga tggtcctatc
      541 atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc cagcgacgga
      601 gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc
      661 caattcaaga ctacttacaa ggcggcaaaa aagattctta aaatgccagg aagccattac
      721 atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt agaagatgca
      781 gtagctcatt ccggcggcat ggacgagctg tacaagtccg gatccaagct gtagcggccg
      841 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      901 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      961 tttattgcag cttataatgg ttacaaataa agca
//
