LOCUS       sequence-84933-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 568, Emission:
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mApple-pBAD
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..80
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(84..92,93..107)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             123..833
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     536..555
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(879..896)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 aggaggaatt aaccatgggg ggttctcatc atcatcatca tcatggtatg gctagcatga
       61 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgagctcga
      121 gcatggtgag caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca
      181 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      241 gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc
      301 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggtc tacattaagc
      361 acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc
      421 gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa ccaggactcc tccctgcagg
      481 acggcgtgtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      541 taatgcagaa gaagaccatg ggctgggagg cctccgagga gcggatgtac cccgaggacg
      601 gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa ggacggcggc cactacgccg
      661 ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacatcg
      721 tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg gaacagtacg
      781 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagaattcg
      841 aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
      901 cgcagaagc
//
