LOCUS       sequence-84937-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..95
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     46..66
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             130..180
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      196..205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             202..915
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
       61 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      121 agcgccacca tgggcgtggc cgacttgatc aagaagttcg agtccatctc caaggaggag
      181 ggggatccac cggtcgccac catggtgagc aagggcgagg ggcaatcgaa gcatgggcta
      241 aaggaggaga tgacggtgaa gtaccacatg gaaggttgcg ttaatggtca caaatttgtc
      301 attactggag agggcattgg aaaccctttt aagggtaagc aaactgcaaa tttgtgtgtg
      361 atagaaggag gcccgctgcc gttctcggag gacattttaa gtccgggttt taaatatggt
      421 gaccggattt tcacagagta cccacaggat attgtagatt acttcaagaa ctcatgtcca
      481 gcgggctata cgtgggaaag gagcttcctc tttgaggacg gagcggtctg tcgatgcaac
      541 gtggacataa cagtctctga aaaggagaac tgcatctatc acaaaagtat cttcagaggg
      601 gtgaattttc ccgccgacgg ccccgtaatg aagaaaatga ccactaattg ggaagctagt
      661 accgagaaaa ttgtgcctgt tccaaagcaa gggatattaa aaggaaaggt caaaatgtac
      721 ctattgctga aggatggcgg tcgttatcat tgccagtttg atacggtata taaagctaaa
      781 tcagtgccct ccaagatgcc agaatggcat tttatacagc ataagctcct tagggaggac
      841 cgctctgatg ctaaaaacca gaaatggcaa ttaacagaac atgcaatcgc aggcatggac
      901 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
      961 ggttttactt gctttaaaaa acctcccaca cctc
//
