LOCUS       sequence-84938-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEL-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..95
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     46..66
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             140..817
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    818..883
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
       61 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      121 agcgctaccg gtcgccacca tgagtgcgat taagccagac atgaagatca aactccgtat
      181 ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt
      241 tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt
      301 tgatatcctg accactgcat tcctgtacgg caacagggta ttcgccaaat atccagacaa
      361 catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac
      421 tttcgaagac gggggcattt gcaacgccag aaacgacata acaatggaag gggacacttt
      481 ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa
      541 gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac
      601 gggtgatgta gagatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag
      661 aactacttac aaagctaagg agaagggtgt caagttacca ggcgcccact ttgtggacca
      721 ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc
      781 tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggactcagat ctcgagctca
      841 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
      901 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      961 cctccccctg aacctgaaac ataaaatgaa tgca
//
