LOCUS       sequence-84939-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 383, Emission:
            448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EBFP2-pBAD
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             88..110
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             130..147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             151..183
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(223..225,226..228,229..942)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(268..289)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atccataaga ttagcggata ctacctgacg ctttttatcg caactctcta ctgtttctcc
       61 atacccgttt tttgggctag aaataatttt gtttaacttt aagaaggaga tatacatatg
      121 cggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      181 ggtcgggatc tgtacgagaa cctgtacttc cagggctcga gcatggtgag caagggcgag
      241 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      301 aagttcagcg tgaggggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
      361 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc
      421 cacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      481 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcacc
      541 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      601 aagggcgtcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc
      661 aacagccaca acatctatat catggccgtc aagcagaaga acggcatcaa ggtgaacttc
      721 aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      781 acccccatcg gcgacggccc cgtgctgctg cccgacagcc actacctgag cacccagtcc
      841 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttccgcacc
      901 gccgccggga tcactctcgg catggacgag ctgtacaagt aagaattcga agcttggctg
      961 ttttggcgga tgagagaaga ttttcagcct gata
//
