LOCUS       sequence-84940-e        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoplasm, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mApple-Display-5
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..802
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     508..527
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             824..970
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 atccgctagc gccaccatgg agaccgacac cctcctgcta tgggtgctgc tgctctgggt
       61 gcccggcagc accggcgacg caccggtcgc caccatggtg agcaagggcg aggagaataa
      121 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      181 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      241 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      301 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      361 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      421 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      481 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      541 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      601 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      661 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      721 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      781 cggcatggac gagctgtaca agtccggaag cggcagcggc ggagctgtgg gccaggacac
      841 gcaggaggtc atcgtggtgc cacactcctt gccctttaag gtggtggtga tctcagccat
      901 cctggccctg gtggtgctca ccatcatctc ccttatcatc ctcatcatgc tttggcagaa
      961 gaagccacgt taggaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta
     1021 gataactgat cataa
//
