LOCUS       sequence-84941-e        1539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: tdEos-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..1539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    5..85
                     /label=MCS
                     /note="multiple cloning site"
     CDS             93..770
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             819..1493
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg
       61 taccgcgggc ccgggatcca ccggtcgcca ccatgagtgc gattaagcca gacatgaaga
      121 tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
      181 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
      241 tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg
      301 aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
      361 aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg
      421 aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc
      481 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
      541 atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat gcccattacc
      601 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc
      661 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
      721 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga tccggtagcg
      781 gtcacggtac tggttctact ggttctggtt cttctgagag tgcgattaag ccagacatga
      841 agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
      901 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
      961 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
     1021 ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
     1081 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
     1141 tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg
     1201 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
     1261 gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt
     1321 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
     1381 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
     1441 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggat
     1501 ccaagctgta gcggccgcga ctctagatca taatcagcc
//
