LOCUS       sequence-84944-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: wtKikGR-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..773
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
     misc_feature    774..839
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
       61 cagatccgct agcgctaccg gtcgccacca tggtgagtgt gattacatca gaaatgaaga
      121 tcgagctgcg tatggaaggc gctgtaaacg ggcacaagtt cgtgattaca gggaaaggaa
      181 gtggccagcc tttcgaggga atacagaatg tggacctgac agtcatagag ggcggacctc
      241 ttccttttgc tttcgatatc ctgacaacag cattccatta cggcaaccgg gtatttgtcg
      301 aatacccaga agaaatagta gactacttca agcagtcgtt tcctgagggt tattcttggg
      361 aacgaagcat gagttacgaa gacgggggaa tttgcctcgc cacaaacaat ataacgatga
      421 agaaagacgg cagcaactgt tttgtcaatg aaattcgatt tgatggtgtg aactttcctg
      481 ccaatggtcc agttatgcag aggaagaccg tcaaatggga gccatccact gagaaaatgt
      541 atgtgcgtga tggagtgctg aagggtgatg taaacatggc tctgttgctt caaggaggtg
      601 gccattaccg atgtgacttc agaactactt acaaagcaaa gaaggttgtc cagttgccag
      661 actatcactt cgtggatcat caaatggaga taacaagcca tgacaaggat tacaacaagg
      721 ttaagctgta tgagcacgct aaagctcatt ccgggctgcc aaggctggcc aagtccggac
      781 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
      841 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      901 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      961 ttaacttgtt tattgcagct tataatggtt acaa
//
