LOCUS       sequence-84947-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 
            516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, 
            Liu Y, Zhang J, Xu P, Xu T
  TITLE     Rational design of true monomeric and bright photoactivatable 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021.
  PUBMED    22581370
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-05-13- 13"; volume: "9"; 
            issue: "7"; pages: "727-9"
COMMENT     Sequence Label: mEos3.2-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             100..777
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    778..843
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tgagtgcgat taagccagac
      121 atgaagatca aactccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga
      181 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc
      241 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta
      301 ttcgccaaat atccagacaa catacaagac tattttaagc agtcgtttcc taaggggtat
      361 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcaacgccag aaacgacata
      421 acaatggaag gggacacttt ctataataaa gttcgatttt atggtaccaa ctttcccgcc
      481 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat
      541 gtgcgtgatg gagtgctgac gggtgatatt gagatggctt tgttgcttga aggaaatgcc
      601 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca
      661 ggcgcccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag
      721 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc
      781 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
      841 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
      901 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      961 gttgttaact tgtttattgc agcttataat ggtt
//
