LOCUS       sequence-84948-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        30..129
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     80..100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..214
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      230..239
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             236..955
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(281..302)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     889..910
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
       61 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      121 agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgggcg tggccgactt
      181 gatcaagaag ttcgagtcca tctccaagga ggagggggat ccaccggtcg ccaccatggt
      241 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      301 cgtaaacggc cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa
      361 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      421 gaccaccttc ggctacggcg tggcctgctt cagccgctac cccgaccaca tgaagcagca
      481 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa
      541 ggacgacggt acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      601 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      661 ggagtacaac ttcaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat
      721 caaggctaac ttcaagatcc gccacaacgt tgaggacggc agcgtgcagc tcgccgacca
      781 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      841 gagccatcag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      901 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc
      961 cgcgactcta gatcataatc agccatacca catgta
//
