LOCUS       sequence-84949-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mRuby-C1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..148
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     99..119
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             193..873
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     misc_feature    874..939
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(1100..1119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
       61 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      121 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      181 ccggtcgcca ccatgaacag cctgatcaaa gaaaacatgc ggatgaaggt ggtgctggaa
      241 ggcagcgtga acggccacca gttcaagtgc accggcgagg gcgagggcaa cccctacatg
      301 ggcacccaga ccatgcggat caaagtgatc gagggcggac ctctgccctt cgccttcgac
      361 atcctggcca catccttcat gtacggcagc cggaccttca tcaagtaccc caagggcatc
      421 cccgatttct tcaagcagag cttccccgag ggcttcacct gggagagagt gaccagatac
      481 gaggacggcg gcgtgatcac cgtgatgcag gacaccagcc tggaagatgg ctgcctggtg
      541 taccatgccc aggtcagggg cgtgaatttt cccagcaacg gcgccgtgat gcagaagaaa
      601 accaagggct gggagcccaa caccgagatg atgtaccccg ctgacggcgg actgagaggc
      661 tacacccaca tggccctgaa ggtggacggc ggagggcacc tgagctgcag cttcgtgacc
      721 acctaccgat ccaagaaaac cgtgggcaac atcaagatgc ccggcatcca cgccgtggac
      781 caccggctgg aaaggctgga agagtccgac aacgagatgt tcgtggtgca gcgggagcac
      841 gccgtggcca agttcgccgg cctgggcgga gggtccggac tcagatctcg agctcaagct
      901 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
      961 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     1021 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     1081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc attttt
//
