LOCUS       sequence-84951-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 610, Emission: 652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mMaroon-N1
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..99
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     50..70
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    122..202
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      204..213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             210..944
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
       61 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      121 cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
      181 cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgatcaagg
      241 agaacatgca caccaagctg tacctgacag gcaccgtgaa caaccactac ttcgaatgca
      301 ccgccgaagg ggagggcaag ccctacgagg gcacccagac caacaggatc aaggtggtgc
      361 gcggaggccc cctgccgttc gcattcgaca tcctggcccc ctgctttatg tacgggagca
      421 agaccttcat taaccatcca cctgacattc ccgattattt taagcagtcc ttccctgagg
      481 gcttcacatg ggagagaacc accgtttacg aagacggggg cacactcact gctacacagg
      541 acaccagcct ccaggacggc tgcctgatct acaacgtcca agtcagaggg gagaacttcc
      601 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccagt accgagaccc
      661 tgtaccccgc tgacggcagc ctggaaggca gactggactg ggccctgaag ctcgtgggcg
      721 ggggccacct gcactgcaga ctggaaacca catacagatc caagaaaccc gcaaagaacc
      781 tcaagatgcc cggcgtctat tttattgaca ggagactgga aagaatcaag gaggccgaca
      841 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
      901 aactggggca caaacttaat ggcatggacg agctgtacaa gtaagcggcc gcgactctag
      961 atcataatca gccataccac
//
