LOCUS       sequence-84952-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 551, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, 
            Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, 
            Masai H, Miyawaki A
  TITLE     Visualizing spatiotemporal dynamics of multicellular cell-cycle 
            progression.
  JOURNAL   Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033.
  PUBMED    18267078
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.12"; journalName: "Cell"; date: "2008-02-8- 8";
            volume: "132"; issue: "3"; pages: "487-98"
COMMENT     Sequence Label: mKO2-pBAD
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..166
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     89..108
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             193..215
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             235..252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             256..288
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             301..321
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             328..984
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtcacatt
       61 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
      121 gatactacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg
      181 gctagaaata attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat
      241 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      301 gagaacctgt acttccaggg ctcgagcatg gtgagtgtga ttaaaccaga gatgaagatg
      361 aggtactaca tggacggctc cgtcaatggg catgagttca caattgaagg tgaaggcaca
      421 ggcagacctt acgagggaca tcaagagatg acactacgcg tcacaatggc cgagggcggg
      481 ccaatgcctt tcgcgtttga cttagtgtca cacgtgttct gttacggcca cagagtattt
      541 actaaatatc cagaagagat accagactat ttcaaacaag catttcctga aggcctgtca
      601 tgggaaaggt cgttggagtt cgaagatggt gggtccgctt cagtcagtgc gcatataagc
      661 cttagaggaa acaccttcta ccacaaatcc aaatttactg gggttaactt tcctgccgat
      721 ggtcctatca tgcaaaacca aagtgttgat tgggagccat caaccgagaa aattactgcc
      781 agcgacggag ttctgaaggg tgatgttacg atgtacctaa aacttgaagg aggcggcaat
      841 cacaaatgcc aaatgaagac tacttacaag gcggcaaaag agattcttga aatgccagga
      901 gaccattaca tcggccatcg cctcgtcagg aaaaccgaag gcaacattac tgagcaggta
      961 gaagatgcag tagctcatag ctaagaattc gaagcttggc tgttttggcg gatgagagaa
     1021 gattttcagc
//
