LOCUS       sequence-84954-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: HcRed1-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..91
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             134..817
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     misc_feature    818..883
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 caaatcaacg ggactttcca aatgtcgtac actcgcccat gacgcaaatg gcggtaggcg
       61 tgtacggtgg gaggtctata taagcagagc tggttagtga ccgtcagatc cgctagcgct
      121 accggtcgcc accatggtga gcggcctgct gaaggagagt atgcgcatca agatgtacat
      181 ggagggcacc gtgaacggcc actacttcaa gtgcgagggc gagggcgacg gcaacccctt
      241 cgccggcacc cagagcatga gaatccacgt gaccgagggc gcccccctgc ccttcgcctt
      301 cgacatcctg gccccctgct gcgagtacgg cagcaggacc ttcgtgcacc acaccgccga
      361 gatccccgac ttcttcaagc agagcttccc cgagggcttc acctgggaga gaaccaccac
      421 ctacgaggac ggcggcatcc tgaccgccca ccaggacacc agcctggagg gcaactgcct
      481 gatctacaag gtgaaggtgc acggcaccaa cttccccgcc gacggccccg tgatgaagaa
      541 caagagcggc ggctgggagc ccagcaccga ggtggtgtac cccgagaacg gcgtgctgtg
      601 cggccggaac gtgatggccc tgaaggtggg cgaccggcac ctgatctgcc accactacac
      661 cagctaccgg agcaagaagg ccgtgcgcgc cctgaccatg cccggcttcc acttcaccga
      721 catccggctc cagatgctgc ggaagaagaa ggacgagtac ttcgagctgt acgaggccag
      781 cgtggcccgg tacagcgacc tgcccgagaa ggccaactcc ggactcagat ctcgagctca
      841 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
      901 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      961 cctccccctg aacctgaaac ataaaatgaa tgca
//
