LOCUS       sequence-84955-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VESS-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..93
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     44..64
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             138..815
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             138..815
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    816..881
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
       61 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      121 cgctaccggt cgccaccatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg
      181 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
      241 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
      301 atatcctgac cactgcattc tcctacggca acagggtatt cgccaaatat ccagacaaca
      361 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
      421 tcgaagacgg gggcatttgc aacgccagaa acgacataac aatggaaggg gacactttct
      481 ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga
      541 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg
      601 gtgatgtaga gatggctttg ttgcttgaag gaaatgccca ttaccgatgt gactccagaa
      661 ctacttacaa agctaaggag aagggtgtca agttaccagg cgcccacttt gtggaccact
      721 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
      781 ttgctcattc tggattgcct gacaatgcca gacgatccgg actcagatct cgagctcaag
      841 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
      901 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      961 tccccctgaa cctgaaacat aaaatgaatg caat
//
