LOCUS       sequence-84956-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EBFP2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     45..65
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..179
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(201..203,204..206,207..920)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(246..267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caacgggact ttccaaaatg tcgtaacaac tccgccccca ttgacgcaaa tgggcggtag
       61 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcgccaccat gggcgtggcc gacttgatca agaagttcga gtccatctcc aaggaggagg
      181 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      241 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg
      301 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      361 tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc
      421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      481 tccaggagcg caccatcttc ttcaaggacg acggcaccta caagacccgc gccgaggtga
      541 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg
      601 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac atctatatca
      661 tggccgtcaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacgtggagg
      721 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      781 tgctgctgcc cgacagccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
      841 agaagcgcga tcacatggtc ctgctggagt tccgcaccgc cgccgggatc actctcggca
      901 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
      961 tgtagaggtt ttacttgctt taaaaaaacc tccc
//
