LOCUS       sequence-84957-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mRuby-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..67
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     18..38
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             126..176
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             198..878
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
       61 cagagctggt ttagtgaacc gtcagatccg ctagcgccac cgacgtcaga tccgctagcg
      121 ccaccatggg cgtggccgac ttgatcaaga agttcgagtc catctccaag gaggaggggg
      181 atccaccggt cgccaccatg aacagcctga tcaaagaaaa catgcggatg aaggtggtgc
      241 tggaaggcag cgtgaacggc caccagttca agtgcaccgg cgagggcgag ggcaacccct
      301 acatgggcac ccagaccatg cggatcaaag tgatcgaggg cggacctctg cccttcgcct
      361 tcgacatcct ggccacatcc ttcatgtacg gcagccggac cttcatcaag taccccaagg
      421 gcatccccga tttcttcaag cagagcttcc ccgagggctt cacctgggag agagtgacca
      481 gatacgagga cggcggcgtg atcaccgtga tgcaggacac cagcctggaa gatggctgcc
      541 tggtgtacca tgcccaggtc aggggcgtga attttcccag caacggcgcc gtgatgcaga
      601 agaaaaccaa gggctgggag cccaacaccg agatgatgta ccccgctgac ggcggactga
      661 gaggctacac ccacatggcc ctgaaggtgg acggcggagg gcacctgagc tgcagcttcg
      721 tgaccaccta ccgatccaag aaaaccgtgg gcaacatcaa gatgcccggc atccacgccg
      781 tggaccaccg gctggaaagg ctggaagagt ccgacaacga gatgttcgtg gtgcagcggg
      841 agcacgccgt ggccaagttc gccggcctgg gcggagggta agcggccgcg actctagatc
      901 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaaacc tcccacacct
      961 ccccctgaac ctgaaacata aaatgaatgc aatt
//
