LOCUS       sequence-84958-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-NT-C1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..67
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     18..38
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    793..858
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
       61 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catgaacagc
      121 ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
      181 ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg gaactcaaac catgaggatc
      241 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
      301 tatggcagcc gtacttttat caagtacccg aaaggcattc ctgatttctt taaacagtcc
      361 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
      421 gtcatgcagg acaccagcct tgaggatggc tgtctcgttt accacgtcca agtcagaggg
      481 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
      541 acagagatga tgtatccagc agatggtggt ctgaggggat acactcatat ggcactgaaa
      601 gttgatggtg gtggccatct gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc
      661 gtcgggaaca tcaagatgcc cggtatccat gccgttgatc accgcctgga aaggttagag
      721 gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg
      781 cttggtggtg ggtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
      841 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca tac
//
