LOCUS       sequence-84959-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..67
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     18..38
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..828
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(157..178)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     765..786
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    829..894
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
       61 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
      121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      181 aacggccaca agttcagcgt ccgcggcgag ggcgagggcg atgccaccaa cggcaagctg
      241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      301 accttcggct acggcgtggc ctgcttcagc cgctaccccg accacatgaa gcagcacgac
      361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctc tttcaaggac
      421 gacggtacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      541 tacaacttca acagccacaa cgtctatatc acggccgaca agcagaagaa cggcatcaag
      601 gctaacttca agatccgcca caacgttgag gacggcagcg tgcagctcgc cgaccactac
      661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      721 catcagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
      841 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
      901 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      961 aacctcccac acctccccct gaacctgaaa cata
//
