LOCUS       sequence-84961-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 590, Emission:
            649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Wang L, Jackson WC, Steinbach PA, Tsien RY
  TITLE     Evolution of new nonantibody proteins via iterative somatic 
            hypermutation.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov
            19.
  PUBMED    15556995
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-11-30"; volume: "101(48)"; pages: 
            "16745-9. Epub 2004 Nov 19"
COMMENT     Sequence Label: mPlum-pBAD
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             175..207
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             220..240
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             247..927
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     648..667
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     842..865
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(973..990)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cacactttgc tatgccatag cattttatcc ataagattag cggatactac tgacgctttt
       61 atcgcaactc tctactgttt ctccataccc gttttttggg ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      181 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      241 tcgagcatgg tgagcaaggg cgaggaggtc atcaaggagt tcatgcgctt caaggagcac
      301 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      361 tacgagggca cccagaccgc caggctgaag gtgaccaagg gtggccccct gcccttcgcc
      421 tgggacatcc tgtcccctca gatcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      481 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      541 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      601 ttcatctaca aggtgaaggt gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      661 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      721 aagggcgaga tgaagatgag gctgaggctg aaggacggcg gccactacga cgccgaggtc
      781 aagaccacct acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc
      841 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc
      901 gagggccgcc actccaccgg cgcctaagaa ttcgaagctt ggctgttttg gcggatgaga
      961 gaagattttc agcctgatac agattaaatc agaacgcaga agcgtctata aacagaacgt
     1021 aactaaggg
//
