LOCUS       sequence-84962-e        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 472, Emission:
            495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: MiCy-pBAD
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             156..173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             177..209
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             222..242
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             249..947
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
     primer_bind     complement(993..1010)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cacactttgc tatgccatag catttttatc catagattag cggatactac ctgacgcttt
       61 ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctagaaat aattttgttt
      121 aactttaaga aggagatata catatgcggg gttctcatca tcatcatcat catggtatgg
      181 ctagcatgac tggtggacag caaatgggtc gggatctgta cgagaacctg tacttccagg
      241 gctcgagcat ggtgtcttat tcaaagcaag gcatcgcaca agaaatgcgg acgaaatacc
      301 gtatggaagg cagtgtcaat ggccatgagt tcacgatcga aggtgtagga actggaaacc
      361 cttacgaagg gaaacagatg tccgaattag tgatcatcaa gcctaaggga aaaccccttc
      421 cattctcctt tgacatactg tcaacagcct ttcaatatgg aaacagatgc ttcacaaagt
      481 accctgccga catgcctgac tatttcaagc aagcattccc agatggaatg tcatatgaaa
      541 ggtcatttct atttgaggat ggaggagttg ctacagccag ctggagcatt cgtctcgaag
      601 gaaattgctt catccacaat tccatctatc atggcgtaaa ctttcccgct gatggacccg
      661 taatgaagaa gcagacaatt ggctgggata agtccttcga aaaaatgagt gtggctaaag
      721 aggtgctaag aggtgatgtg actcagtttc ttctgctcga aggaggtggt taccagagat
      781 gccggtttca ctccacttac aaaacggaga agccagtcgc aatgcccccg agtcatgtcg
      841 tagaacatca aattgtgagg accgaccttg gccaaactgc aaaaggcttc aaggtcaagc
      901 tggaagaaca tgctgaggct catgttaacc ctttgaaggt taaataagaa ttcgaagctt
      961 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
     1021 agcggtcgta cc
//
