LOCUS       sequence-84963-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-N1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..104
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     55..75
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    127..207
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      209..218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             215..925
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 caccaaaatc aacgggactt tcccaaaatg tcgtaacaac tccgccccca ttgacgcaaa
       61 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      121 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
      181 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg tctaagggcg aagagctgat
      241 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
      301 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      361 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
      421 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
      481 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      541 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      601 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
      661 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
      721 gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa
      781 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
      841 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
      901 tagcaaactg gggcacaagc ttaattaagc ggccgcgact ctagatcata atcagccata
      961 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1021 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcag
//
