LOCUS       sequence-84970-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 553, Emission: 574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, 
            Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, 
            Chudakov DM
  TITLE     Bright monomeric red fluorescent protein with an extended 
            fluorescence lifetime.
  JOURNAL   Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.
  PUBMED    17572680
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2007-07"; volume: "4(7)"; pages: "555-7. Epub 2007
            Jun 17"
COMMENT     Sequence Label: TurboRFP-N1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..56
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     7..27
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    79..159
                     /label=MCS
                     /note="multiple cloning site"
     CDS             167..862
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
       61 tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat
      121 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatga gcgagctgat
      181 caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
      241 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt
      301 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      361 cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
      421 tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac
      481 ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa
      541 cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga
      601 gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt
      661 gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa
      721 gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc
      781 cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc
      841 tagcaaactg gggcacagat gatgcggccg cgactctaga tcataatcag catacacatt
      901 tgtagaggtt tacttgctta aaa
//
