LOCUS       sequence-84975-e        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 400 / 485, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PA-sfGFP-C1
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        89..140
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     91..111
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(185..187,188..190,191..901)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(230..251)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     838..859
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    902..967
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1088..1167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1123..1142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccaagtctcc accccatgac gtcaatggga gttgtttggc accaaaatca acgggacttc
       61 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      121 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggtcgc
      181 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      241 ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac
      301 caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      361 caccctcgtg accaccctga gctacggcgt gcagtgcttc agccgctacc ccgaccacat
      421 gaagcgccac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      481 cagcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac
      541 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      601 gcacaagctg gagtacaact tcaacagcca caacgtctat atcaccgccg acaagcagaa
      661 gaacggcatc aaggccaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct
      721 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      781 ccactacctg agccaccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat
      841 ggtcctgctg gagttcgtga ccgccgccgg gatcactcac ggcatggacg agctgtacaa
      901 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc
      961 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     1021 acttgcttta aaaacctccc acacctcccc ctgaacctga aacataaatg aatgcattgt
     1081 tgttgttaac ttgtttattg cagcttataa tgttacaata aagcaatagc atcacaaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg
//
