LOCUS       sequence-84976-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Pedelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS
  TITLE     Engineering and characterization of a superfolder green fluorescent 
            protein.
  JOURNAL   Nat Biotechnol. 2006 Jan;24(1):79-88. doi: 10.1038/nbt1172
  PUBMED    16369541
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2006-01"; volume: "24"; issue: "1"; pages: 
            "79-88"
COMMENT     Sequence Label: sfGFP-C1
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..83
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     34..54
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(128..130,131..133,134..844)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(173..194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     781..802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    845..910
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
       61 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt
      121 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      181 gctggacggc gacgtaaacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgatgc
      241 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      361 catgaagcgc cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      421 catcagcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga
      481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      541 ggggcacaag ctggagtaca acttcaacag ccacaacgtc tatatcaccg ccgacaagca
      601 gaagaacggc atcaaggcca acttcaagat ccgccacaac gtggaggacg gcagcgtgca
      661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      721 caaccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca
      781 catggtcctg ctggagttcg tgaccgccgc cgggatcact cacggcatgg acgagctgta
      841 caagtccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg
      901 cccgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
      961 tttacttgct ttaaaaa
//
