LOCUS       sequence-84977-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mDsRed-N1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..143
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     94..114
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    166..246
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      248..257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             254..931
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     652..671
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    1053..1132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ccaagtctcc accccattga cgtcaatggg agtttgtttg gcaccaaaat caacgggact
       61 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      121 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga
      181 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
      241 accggtcgcc accatggaca acaccgagga cgtcatcaag gagttcatgc agttcaaggt
      301 gcgcatggag ggctccgtga acggccacta cttcgagatc gagggcgagg gcgagggcaa
      361 gccctacgag ggcacccaga ccgccaagct gcaggtgacc aagggcggcc ccctgccctt
      421 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggcctacg tgaagcaccc
      481 cgccgacatc cccgactaca tgaagctgtc cttccccgag ggcttcacct gggagcgctc
      541 catgaacttc gaggacggcg gcgtggtgga ggtgcagcag gactcctccc tgcaggacgg
      601 caccttcatc tacaaggtga agttcaaggg cgtgaacttc cccgccgacg gccccgtaat
      661 gcagaagaag actgccggct gggagccctc caccgagaag ctgtaccccc aggacggcgt
      721 gctgaagggc gagatctccc acgccctgaa gctgaaggac ggcggccact acacctgcga
      781 cttcaagacc gtgtacaagg ccaagaagcc cgtgcagctg cccggcaacc actacgtgga
      841 ctccaagctg gacatcacca accacaacga ggactacacc gtggtggagc agtacgagca
      901 cgccgaggcc cgccactccg gctcccagta agcggccgcg actctagatc ataatcagcc
      961 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1021 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1081 acaaataaag caatagcatc acaatttcac aataaagcat ttttttcact gcattc
//
