LOCUS       sequence-84978-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mRuby-N1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..136
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     87..107
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    159..239
                     /label=MCS
                     /note="multiple cloning site"
     CDS             247..927
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     polyA_signal    1052..1132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1089..1108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
       61 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      121 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
      181 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
      241 gccaccatga acagcctgat caaagaaaac atgcggatga aggtggtgct ggaaggcagc
      301 gtgaacggcc accagttcaa gtgcaccggc gagggcgagg gcaaccccta catgggcacc
      361 cagaccatgc ggatcaaagt gatcgagggc ggacctctgc ccttcgcctt cgacatcctg
      421 gccacatcct tcatgtacgg cagccggacc ttcatcaagt accccaaggg catccccgat
      481 ttcttcaagc agagcttccc cgagggcttc acctgggaga gagtgaccag atacgaggac
      541 ggcggcgtga tcaccgtgat gcaggacacc agcctggaag atggctgcct ggtgtaccat
      601 gcccaggtca ggggcgtgaa ttttcccagc aacggcgccg tgatgcagaa gaaaaccaag
      661 ggctgggagc ccaacaccga gatgatgtac cccgctgacg gcggactgag aggctacacc
      721 cacatggccc tgaaggtgga cggcggaggg cacctgagct gcagcttcgt gaccacctac
      781 cgatccaaga aaaccgtggg caacatcaag atgcccggca tccacgccgt ggaccaccgg
      841 ctggaaaggc tggaagagtc cgacaacgag atgttcgtgg tgcagcggga gcacgccgtg
      901 gccaagttcg ccggcctggg cggagggtaa gcggccgcga ctctagatca taatcagcca
      961 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     1021 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     1081 caaataaagc aatagcatca caaatttcac aaataaagca ttttttcact gcattc
//
