LOCUS       sequence-84982-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTagRFPet-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..57
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     8..28
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..833
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    834..899
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
       61 ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgtct aagggcgaag
      121 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
      181 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      241 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
      301 tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgatttc tttaagcagt
      361 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
      421 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
      481 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca
      541 acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac atggccctga
      601 agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac
      661 ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca
      721 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
      781 acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac aagtccggac
      841 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
      901 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      961 taaaaaacct cccacacctc cccctgaacc tgaa
//
