LOCUS       sequence-84984-e        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY
  TITLE     Reducing the environmental sensitivity of yellow fluorescent 
            protein. Mechanism and applications.
  JOURNAL   J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31.
  PUBMED    11387331
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-08-3"; volume: "276(31)"; pages: "29188-94. Epub
            2001 May 31"
COMMENT     Sequence Label: mCitrine-C1
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..128
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     79..99
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      167..176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(173..175,176..178,179..889)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(218..239)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(479..498)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     826..847
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    890..955
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 cccatgacgt caatggagtt gtttggcacc aaaatcaacg ggactttcca aaatgtcgta
       61 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      121 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
      181 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      241 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      301 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      361 caccttcggc tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga
      421 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      481 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      541 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      601 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      661 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      721 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      781 ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      841 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag
      901 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
      961 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1021 aacctcccac acctccccct gaacctgaac ataaatgaat gcattgttgt tgtaacttgt
     1081 tatgcagctt atatggttac aataagcata gcatcacaaa tttcacaata agcatttttt
     1141 cactgcattc tagtgtggtt gtccaactca tcatgatcta cgcgtaaatg
//
