LOCUS       sequence-84986-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VESS-N1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..58
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     9..29
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    81..161
                     /label=MCS
                     /note="multiple cloning site"
     CDS             169..849
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             169..849
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
       61 tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga
      121 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat gagtgcgatt
      181 aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
      241 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc
      301 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ctcctacggc
      361 aacagggtat tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct
      421 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caacgccaga
      481 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
      541 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
      601 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgtag agatggcttt gttgcttgaa
      661 ggaaatgccc attaccgatg tgactccaga actacttaca aagctaagga gaagggtgtc
      721 aagttaccag gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat
      781 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
      841 agacgataag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
      901 acttgcttta aaaaacctcc cac
//
