LOCUS       sequence-84987-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..58
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     9..29
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    81..161
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      163..172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             169..903
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
       61 tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga
      121 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
      181 ggcgaggagc tgatcaagga gaacatgcac atgaagctgt acatggaagg caccgtgaac
      241 aaccaccact tcaagtgcac caccgaaggg gagggcaagc cctacgaggg cacccagacc
      301 cagaggatta aggtggtgga gggaggcccc ctgccgttcg cattcgacat cctggccacc
      361 tgctttatgt acgggagcaa gaccttcatc aaccacaccc agggcatccc cgatttcttt
      421 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      481 gtgcttaccg ttacccagga caccagcctc caggacggct gcttgatcta caacgtcaag
      541 ctcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      601 gaggccacca ccgagaccct gtaccccgct gacggcggcc tggaaggcag atgcgacatg
      661 gccctgaagc tcgtgggcgg gggccacctg cactgcaacc tgaagaccac atacagatcc
      721 aagaaacccg ctaagaacct caagatgccc ggcgtctact ttgtggaccg cagactggaa
      781 agaatcaagg aggccgacaa tgagacctac gtcgagcagc acgaggtggc tgtggccaga
      841 tactgcgacc tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag
      901 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
      961 tttaaaaaac ctcccacacc tccccctgaa cctg
//
