LOCUS       sequence-84988-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 450, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: iLOV-C1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..132
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     83..103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    510..575
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    699..820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(736..755)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     790..809
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
       61 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      121 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatga
      181 tcgagaagaa cttcgtgatc accgacccca gactgcccga caaccccatc atcttcgcca
      241 gcgacggctt cctggagctg accgagtaca gcagagagga gatcctgggc agaaacgcca
      301 gattcctgca gggccccgag accgaccagg ccaccgtgca gaagatcaga gacgccatca
      361 gagaccagag agagaccacc gtgcagctga tcaactacac caagagcggc aagaagttct
      421 ggaacctgct gcacctgcag cccgtgagag accagaaggg cgagctgcag tacttcatcg
      481 gcgtgcagct ggacggcagc gaccacgtgt ccggactcag atctcgagct caagcttcga
      541 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
      601 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      661 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      781 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
      841 aatattttgt ta
//
