LOCUS       sequence-84990-e        1172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY
  TITLE     Reducing the environmental sensitivity of yellow fluorescent 
            protein. Mechanism and applications.
  JOURNAL   J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31.
  PUBMED    11387331
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-08-3"; volume: "276(31)"; pages: "29188-94. Epub
            2001 May 31"
COMMENT     Sequence Label: Citrine-N1
FEATURES             Location/Qualifiers
     source          1..1172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..130
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     81..101
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    153..233
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      235..244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(241..243,244..246,247..957)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(286..307)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(547..566)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     894..915
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1082..1162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1119..1138)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccccatgacg tcaatgggag ttgtttggca ccaaaatcaa cgggactttc caaaatgtcg
       61 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      121 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
      181 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      241 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      301 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      361 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      421 ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag
      481 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      541 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      601 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      661 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      721 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      781 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      841 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      901 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      961 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1021 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     1081 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1141 aaataagcat ttttttcact gcattctagt tg
//
