LOCUS       sequence-84993-e        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mOrange2-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..133
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     84..104
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    156..236
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      238..247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             244..951
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    1088..1168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1124..1143)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cccccattga cgtcaatggg agtttgtttg gcaccaaaat caacgggact ttccaaaatg
       61 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      121 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
      181 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
      241 accatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc
      301 aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      361 ggccgcccct acgagggctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg
      421 cccttcgcct gggacatcct gtcccctcat ttcacctacg gctccaaggc ctacgtgaag
      481 caccccgccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caagtgggag
      541 cgcgtgatga actacgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      601 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      661 gtgatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      721 ggtgccctga agggcaagat caagatgagg ctgaagctga aggacggcgg ccactacacc
      781 tccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
      841 gtcgacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      901 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtccggatcc
      961 aagctgtagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     1021 cttgctttaa aaacctccca cacctccccc tgaacctgaa acataaatga atgcaattgt
     1081 tgttgttaac ttgtttattg cagcttataa tggttacaat aaagcaatag catcacaaat
     1141 ttcacaaata aagcattttt ttcactgcat tctagttg
//
