LOCUS       sequence-85004-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 475, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AcGFP1-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..61
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     12..32
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..822
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    823..888
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
       61 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
      121 gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc tgaatggcga tgtgaatggc
      181 cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      241 aagttcatct gcaccaccgg caagctgcct gtgccctggc ccaccctggt gaccaccctg
      301 agctacggcg tgcagtgctt ctcacgctac cccgatcaca tgaagcagca cgacttcttc
      361 aagagcgcca tgcctgaggg ctacatccag gagcgcacca tcttcttcga ggatgacggc
      421 aactacaagt cgcgcgccga ggtgaagttc gagggcgata ccctggtgaa tcgcatcgag
      481 ctgaccggca ccgatttcaa ggaggatggc aacatcctgg gcaataagat ggagtacaac
      541 tacaacgccc acaatgtgta catcatgacc gacaaggcca agaatggcat caaggtgaac
      601 ttcaagatcc gccacaacat cgaggatggc agcgtgcagc tggccgacca ctaccagcag
      661 aataccccca tcggcgatgg ccctgtgctg ctgcccgata accactacct gtccacccag
      721 agcgccctgt ccaaggaccc caacgagaag cgcgatcaca tgatctactt cggcttcgtg
      781 accgccgccg ccatcaccca cggcatggat gagctgtaca agtccggact cagatctcga
      841 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga
      901 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      961 ccacacctcc ccctgaacct gaaacataaa atga
//
