LOCUS       sequence-85005-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 558, Emission:
            583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DsRed2-pBAD
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..37
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             121..143
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             163..180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..216
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             229..249
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             256..933
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(436..456)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     848..871
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atactactga
       61 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
      121 tttgtttaac tttaagaagg agatatacat atgcggggtt ctcatcatca tcatcatcat
      181 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga gaacctgtac
      241 ttccagggct cgagcatggc ctcctccgag aacgtcatca ccgagttcat gcgcttcaag
      301 gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      361 cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc
      421 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac
      481 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      541 gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc cctgcaggac
      601 ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgtg
      661 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
      721 gtgctgaagg gcgagaccca caaggccctg aagctgaagg acggcggcca ctacctggtg
      781 gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg
      841 gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag
      901 cgcaccgagg gccgccacca cctgttcctg taagaattcg aagcttggct gttttggcgg
      961 atgagagaag attttcagcc tgatacagat taaa
//
