LOCUS       sequence-85006-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 472, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: MiCy-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..89
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     40..60
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             134..829
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
     misc_feature    830..895
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 cgggactttc caaaatgtcg taacaactcc gcccattgac gcaaatgggc ggtaggcgtg
       61 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      121 accggtcgcc accatggtgt cttattcaaa gcaaggcatc gcacaagaaa tgcggacgaa
      181 ataccgtatg gaaggcagtg tcaatggcca tgagttcacg atcgaaggtg taggaactgg
      241 aaacccttac gaagggaaac agatgtccga attagtgatc atcaagccta agggaaaacc
      301 ccttccattc tcctttgaca tactgtcaac agcctttcaa tatggaaaca gatgcttcac
      361 aaagtaccct gccgacatgc ctgactattt caagcaagca ttcccagatg gaatgtcata
      421 tgaaaggtca tttctatttg aggatggagg agttgctaca gccagctgga gcattcgtct
      481 cgaaggaaat tgcttcatcc acaattccat ctatcatggc gtaaactttc ccgctgatgg
      541 acccgtaatg aagaagcaga caattggctg ggataagtcc ttcgaaaaaa tgagtgtggc
      601 taaagaggtg ctaagaggtg atgtgactca gtttcttctg ctcgaaggag gtggttacca
      661 gagatgccgg tttcactcca cttacaaaac ggagaagcca gtcgcaatgc ccccgagtca
      721 tgtcgtagaa catcaaattg tgaggaccga ccttggccaa actgcaaaag gcttcaaggt
      781 caagctggaa gaacatgctg aggctcatgt taaccctttg aaggttaaat ccggactcag
      841 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
      901 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      961 aaacctccca cacctccccc tgaacctgaa acat
//
