LOCUS       sequence-85010-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-C1
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..111
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     62..82
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             156..863
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     misc_feature    864..929
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
       61 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      121 aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagacca
      181 caatgggcgt aatcaagccc gacatgaaga tcaagctgaa gatggagggc aacgtgaatg
      241 gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc accaacacca
      301 tcaacctgga ggtgaaggag ggagcccccc tgcccttctc ctacgacatt ctgaccaccg
      361 cgttcgccta cggcaacagg gccttcacca agtaccccga cgacatcccc aactacttca
      421 agcagtcctt ccccgagggc tactcttggg agcgcaccat gaccttcgag gacaagggca
      481 tcgtgaaggt gaagtccgac atctccatgg aggaggactc cttcatctac gagatacacc
      541 tcaagggcga gaacttcccc cccaacggcc ccgtgatgca gaagaagacc accggctggg
      601 acgcctccac cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac gtcaagcaca
      661 agctgctgct ggagggcggc ggccaccacc gcgttgactt caagaccatc tacagggcca
      721 agaaggcggt gaagctgccc gactatcact ttgtggacca ccgcatcgag atcctgaacc
      781 acgacaagga ctacaacaag gtgaccgttt acgagagcgc cgtggcccgc aactccaccg
      841 acggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg
      901 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
      961 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1021 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1081 acaaataaag caatagcatc acaa
//
