LOCUS       sequence-85011-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..72
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     23..43
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    95..175
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      177..186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             183..893
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
       61 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg
      121 agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
      181 ccatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag gtggtcatgg
      241 aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc aatccgtaca
      301 tgggaactca aaccatgagg atcaaagtca tcgagggagg acccctgcca tttgcctttg
      361 acattcttgc cacgtcgttc atgtatggca gccgtacttt tatcaagtac ccgaaaggca
      421 ttcctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga gttacgagat
      481 acgaagatgg tggagtcgtc accgtcatgc aggacaccag ccttgaggat ggctgtctcg
      541 tttaccacgt ccaagtcaga ggggtaaact ttccctccaa tggtcccgtg atgcagaaga
      601 agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt ggtctgaggg
      661 gatacactca tatggcactg aaagttgatg gtggtggcca tctgtcttgc tctttcgtaa
      721 caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtatc catgccgttg
      781 atcaccgcct ggaaaggtta gaggaaagtg acaatgaaat gttcgtagta caacgcgaac
      841 acgcagttgc caagttcgcc gggcttggtg gtgggatgga cgagctgtac aagtaagcgg
      901 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      961 aacctcccac acctccccct gaacctgaaa cata
//
