LOCUS       sequence-85020-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 514, Emission:
            527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Topaz-pBAD
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..201
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             214..234
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(241..243,244..246,247..960)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(286..307)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(547..566)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     894..915
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatgccata gcatttttat ccataagatt agcggatact acctgacgct tttatcgcaa
       61 ctctctactg tttctccata cccgtttttt tgggctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
      181 actggtggac agcaaatggg tcgggatctg tacgagaacc tgtacttcca gggctcgagc
      241 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      301 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      361 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      421 ctcgtgacca ccttcggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgcgc
      481 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      541 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      601 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      661 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      721 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      781 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      841 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      901 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      961 gaattcgaag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa
     1021 a
//
