LOCUS       sequence-85021-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 490 / 553, Emission: 
            507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dendra1-N1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..157
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     108..128
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    180..260
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      262..271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
       61 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      121 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      181 ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
      241 cgggcccggg atccaccggt cgccaccatg gacccgggta ccgtcaatct gattaaagaa
      301 gatatgaggg ttaaggtgca tatggaaggg aatgtaaacg ggcatgcttt tgtgattgaa
      361 ggggaaggaa aaggaaagcc ctacgaaggg acacagaccg cgaacctgac agtgaaagaa
      421 ggcgcgcctc tcccattttc ttacgacatc ttgacaacag cagtgcacta cggaaacaga
      481 gtattcacca aataccctga agatataccg gattatttca agcagtcgtt tcctgaagga
      541 tattcctggg aaagaaccat gacttttgaa gacaagggca tttgtaccat cagaagcgac
      601 ataagcttgg aaggtgactg ctttttccaa aacgttcgtt ttaaggggac caactttccc
      661 ccaaatggtc cagttatgca gaagaaaact ttgaagtggg aaccatccac agagaagctg
      721 cacgtgcgtg atgggttgct tgtcggtaat attaacatgg ctctgctgct tgaaggaggt
      781 ggacattacc tgtgtgactt caaaactact tacaaagcga agaaggttgt tcagttgcca
      841 gatgcccatt ttgtggacca tcgcattgag atcttgggta atgacagcga ttacaacaaa
      901 gtgaagctgt acgagcatgc cgttgctcgc tattctccgt tgccaagtca ggcctggtag
      961 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1021 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatga
//
