LOCUS       sequence-85024-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mApple-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..94
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..809
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     515..534
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gaacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
       61 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
      121 agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      181 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gaggcctttc
      241 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      301 cccctcagtt catgtacggc tccaaggtct acattaagca cccagccgac atccccgact
      361 acttcaagct gtccttcccc gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg
      421 gcggcattat tcacgttaac caggactcct ccctgcagga cggcgtgttc atctacaagg
      481 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      541 gctgggaggc ctccgaggag cggatgtacc ccgaggacgg cgccctgaag agcgagatca
      601 agaagaggct gaagctgaag gacggcggcc actacgccgc cgaggtcaag accacctaca
      661 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag ttggacatcg
      721 tgtcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      781 ccaccggcgg catggacgag ctgtacaagt ccggatccaa gctgtagcgg ccgcgactct
      841 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
      901 acctccccct gaacctgaaa cat
//
