LOCUS       sequence-85025-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mPA-GFP-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..52
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     3..23
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    75..155
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(163..165,166..168,169..879)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(208..229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(469..488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     816..837
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
       61 gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
      121 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      181 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      241 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      301 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcagc
      361 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      421 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      481 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      541 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      601 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggccaacttc
      661 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      721 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ccaccagtcc
      781 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      841 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggatccaa gctgtagcgg
      901 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      961 aaacctccca cacctccccc ctgaacctga aacataaaat gaatgcaatt gttgttgtt
//
