LOCUS       sequence-85026-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mPlum-N1
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..52
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     3..23
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    75..155
                     /label=MCS
                     /note="multiple cloning site"
     CDS             163..843
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     564..583
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     758..781
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
       61 gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
      121 cagtcgacgg taccgcgggc ccgggatcca ccggtggcta ccatggtgag caagggcgag
      181 gaggtcatca aggagttcat gcgcttcaag gagcacatgg agggctccgt gaacggccac
      241 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccagg
      301 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagatc
      361 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      421 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      481 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaaggtgcgc
      541 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      601 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatgaa gatgaggctg
      661 aggctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag
      721 cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac
      781 gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc
      841 taagcggccg cgactctaga tcataatcag ccataccaca tttgtaga
//
