LOCUS       sequence-85029-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoplasm, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Display-5
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..780
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             802..948
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggag accgacaccc tcctgctatg ggtgctgctg
       61 ctctgggtgc ccggcagcac cggcgacgca ccggtcgcca ccatgagtgc gattaagcca
      121 gacatgaaga tcaaactccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac
      181 ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag
      241 ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg
      301 gtattcgcca aatatccaga caacatacaa gactatttta agcagtcgtt tcctaagggg
      361 tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac
      421 ataacaatgg aaggggacac tttctataat aaagttcgat tttatggtac caactttccc
      481 gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg
      541 tatgtgcgtg atggagtgct gacgggtgat attcatatgg ctttgttgct tgaaggaaat
      601 gcccattacc gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta
      661 ccaggctacc actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac
      721 aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga
      781 tccggaagcg gcagcggcgg agctgtgggc caggacacgc aggaggtcat cgtggtgcca
      841 cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc
      901 atcatctccc ttatcatcct catcatgctt tggcagaaga agccacgtta ggaattctgc
      961 agtcgacggt accgcgggcc cggg
//
